Structural Determination of the novel Universal Stress Protein G4LZI3 from Shistosoma mansoni using X-ray Crystallography
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Date
2020
Authors
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University of Zululand
Abstract
Structural determination of the novel Universal Stress Protein G4LZI3 from Schistosoma mansoni using X-ray Crystallography
Priscilla Masamba
PhD (Biochemistry) thesis, Department of Biochemistry and Microbiology, Faculty of Science and Agriculture, University of Zululand
Schistosoma mansoni is a parasitic multicellular helminth of the Schistosomatidae family and is
one of the three main causative agents responsible for the multifactorial disease schistosomiasis,
a debilitating and chronic vascular infection of paramount socio-economic importance and
international public health concern. Recent estimates approximate the disease to account for the
loss of 70 million disability-adjusted life years (DALYs) thus acknowledging schistosomiasis a
more debilitating infection than malaria and a huge competitor with HIV/AIDS and TB.
Mortality rates result in nothing less than 250 000 deaths per annum in sub-Saharan Africa alone
where more than 90% of all schistosome infections stem from. Treatment for the disease has now
become an issue of gross concern worldwide without the development of a vaccine as well as
growing evidence of drug resistant strains against Praziquantel, which has been the backbone of
treatment for all schistosome strains for over three decades. Furthermore, the elusiveness
surrounding the mechanism of action of Praziquantel (PZQ), its lack of success in killing larval
schistosomes 2-4 weeks post-infection as well as its failure to disrupt the transmission cycle of
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the worm, has thereby caused wide interest in the design and development of alternative
treatment against schistosomiasis.
It has been proposed that the cascade of events encompassing the transition of the worm
throughout its developmental cycle, leading to the various changes in its morphological and
chemical composition allowing the worm evade the various defense mechanism of humans, as
well as its tolerance to sudden environmental changes and conditions of stress, is to a large
extend due the over-expression of heat shock proteins (HSPs) and universal stress proteins
(USPs). This current study therefore focuses on the G4LZI3 USP whose over-expression has
been displayed throughout all developmental forms of the parasite during conditions of stress
and has therefore been identified as a ‘lead’ molecule in the design of alternative treatment
against schistosomiasis. However, the mechanism of action of USPs is not well understood.
Therefore, the main aim of this study was to determine the structure and infer biological function
of this important druggable protein.
Before the structure was solved, a wide range of polyphenols, which have been proposed as
potential inhibitors were docked onto the homology model of the protein and subjected to
molecular dynamics (MD) simulations. Results revealed strong interaction between two
polyphenols namely curcumin and catechin, suggesting the inhibitory potential of these
polyphenols. The ability of polyphenols to scavenge oxidative stress associates these compounds
with the prevention of diseases or infections that are linked to ROS production, which is one of
the features of schistosomiasis. Hence, investigating polyphenols as potential nutraceuticals for
schistosomiasis may be an interesting venture towards alternative treatment.
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The structure of the G4LZI3 protein was thereafter solved using X-ray crystallography; the
protein consists of 4 parallel β-sheets and 3 α-helices with the presence of a short 310 helix
positioned close to the C-terminal of the protein. A generated Ramachandran plot showed that
though there is still room for improvement, the solved structure is a relatively good model with
92% of the residues present in the favored and allowed regions. Inferred function points towards
the protein binding ATP for regulation or even acting as an ATPase.
Finally, another set of molecular dynamic simulations were conducted on small molecule
inhibitors docked onto the solved structure of the G4LZI3 protein using a pharmacophore
generated from curcumin. Two of the molecules identified exhibited inhibitory activity against
the protein by demonstrating future studies would therefore consider synthesizing these
compounds for both in vivo and in vitro studies towards alternative anti-schistosomidals.
Description
A thesis submitted in fulfillment of the academic requirements for the degree of Doctor of Philosophy in the Department of Biochemistry and Microbiology, Faculty of Science, Agriculture and Engineering, University of Zululand, 2020.
Keywords
novel Universal Stress Protein, G4LZI3, Shistosoma mansoni, X-ray Crystallography